Faculty of Veterinary Medicine, Urmia University Food Safety and Packaging 3092-7668 1 1 2025 05 15 Antioxidant and antifungal properties of methanolic and aqueous extracts of Satureja hortensis and Cinnamomum zeylanicum 15 23 121667 10.30466/fsp.2025.56070.1007 EN Soghra Valizadeh Department of Food Hygiene and Aquatic, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran 0000-0003-0383-7722 Farzad Katiraee Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Hamed Behniafar Department of Medical Parasitology, Sarab Faculty of Medical Sciences, Sarab, Iran Journal Article 2025 03 19 Plant-derived compounds possess robust antioxidant capabilities and exhibit notable antimicrobial activities against diverse pathogens. This study investigated the antioxidant activities of methanolic and aqueous extracts of <em>Satureja hortensis</em> (<em>S. hortensis</em>) and <em>Cinnamomum zeylanicum</em> (<em>C. zeylanicum</em>). The minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of the extracts were also evaluated. The antifungal activity of methanolic extract of <em>S. hortensis</em> (MIC 1.25 mg/mL) was higher than that of the aqueous extract (MIC 5 mg/mL) on <em>P. chrysogenum</em>. For <em>Candida </em>species, especially <em>C. albicans</em>, MIC and MFC of methanolic extract were lower (MIC 0.31 mg/mL, MFC 1.25 mg/mL) than aqueous extract. The methanolic extract of <em>C. zeylanicum</em> exhibited a MIC of 0.31 mg/mL and MFC of 0.62 mg/mL against <em>P. chrysogenum</em>, outperforming the aqueous extract (MIC 0.62 mg/mL, MFC 1.25 mg/mL). Similarly, methanolic extracts showed markedly lower MIC values than the aqueous extracts against <em>Candida </em>species. In antioxidant activity of methanolic and aqueous extracts of both plants, ABTS values were consistently higher than DPPH results, likely due to ABTS's broader sensitivity to hydrophilic and lipophilic antioxidants. In the DPPH assay, the methanolic extract of <em>C. zeylanicum</em> exhibited the highest antioxidant activity with a scavenging percentage of 89.16 ± 0.6%. In the ABTS method, the methanolic extract of <em>C. zeylanicum</em> exhibited highest antioxidant activity, with a scavenging percentage of 97.8 ± 0.1%. The results of this study indicate that <em>S. hortensis</em> and <em>C. zeylanicum</em> extracts are promising natural antifungals and antioxidants. https://fsp.urmia.ac.ir/article_121667_f9ad7f7618d023d18eee52b4b89bcdf3.pdf