Food Safety and Packaging

Abstract Among dairy foods, ice cream has emerged as an attractive carrier for bioactive ingredients due to its widespread consumer acceptance, favorable sensory characteristics, and protective matrix. This review provides an overview of the evolution of biotic ingredients in ice cream, highlighting the transition from conventional probiotic formulations to next-generation postbiotic-based products. Probiotic strains, particularly Lactobacillus and Bifidobacterium spp., have been incorporated into ice cream and shown satisfactory survival during frozen storage, with potential benefits for gut health and immune regulation. Prebiotics, including inulin, fructooligosaccharides, galactooligosaccharides, and dietary fibers, have been used to support microbial viability and functionality, contributing to the development of synbiotic ice cream with improved technological and health-promoting properties. Recent research has increasingly focused on postbiotics, comprising organic acids, bioactive peptides, exopolysaccharides, and microbial cell components.​ Unlike probiotics, postbiotics do not require viability to exert biological effects, offering advantages in terms of stability, safety, and processing tolerance. This review also discusses the effects of biotics on the rheological behavior, texture, melting resistance, and sensory properties of ice cream, as well as their potential health benefits.

Abstract Microbial proliferation and lipid oxidation are major causes of food loss and foodborne risk. Regulatory reassessments and consumer concerns have increasingly restricted the use of various synthetic preservatives and antioxidants. Consequently, plant-derived bioactive agents, including essential oils (EOs) and phenolic compounds, and green synthesized metal and metal oxide nanoparticles (NPs) have emerged as complementary candidates for food preservation and active packaging. This review integrates antimicrobial and antioxidant mechanisms with a bibliometrically supported mapping of the food packaging literature (SCOPUS, engineering domain; 284 keywords, 10 clusters; VOSviewer). Network analysis identified biodegradable matrices (chitosan, gelatin, starch, pectin, and alginate), antimicrobial and antioxidant activity, essential oils, plant-derived compounds such as curcumin and carvacrol, and "active packaging," strongly associated with electrospinning as a prominent manufacturing approach, as the central theme. Separate clusters highlighted sustainability and barrier performance. The review also examines translational limitations, particularly migration, toxicology, and regulatory uncertainty. Bulk transfer from packaging to food depends on polymer embedding, food acidity, and thermal history; however, current evidence often fails to distinguish the release of particulate nanoparticles from oxidative dissolution to ionic species, despite their differing toxicological and regulatory effects. Silver toxicology continues to focus predominantly on ionic behavior, contributing to ongoing uncertainty in European safety assessments. This study reveals that the main limitations stem from reproducibility and regulatory fit rather than efficacy; the primary problems are variability in plant extracts, unstandardized NP characterization, discrepancies between in vitro and real food performance, and the lack of nano-specific regulatory processes.

Abstract Street-vended urojo is one of the most popular ready-to-eat foods in Zanzibar. However, poor food handling practices may increase the risk of food-borne diseases. This research evaluated the level of food safety knowledge and hygienic practices of Urojo Vendors in Urban West Zanzibar Region. A cross-sectional study was conducted among 90 Urojo vendors selected using a non-probability convenience sampling technique. Socio-demographic data were obtained by a pre-prepared questionnaire focusing on knowledge and practices related to food safety and hygiene. Scores for knowledge and practice were classified as low, moderate or high. Pearson’s correlation was utilized to examine the relationship between knowledge and hygiene practice scores, whereas chi-squared tests were employed to determine associations between selected vendor characteristics and hygiene practices. Statistical significance was defined as p <.05. Nearly half of the vendors (46.7%) exhibited high levels of hygiene compared to moderate (36.7%) and low (16.7%). There was a positive correlation (r = 0.563, p <.001) between food safety knowledge and hygiene practice. . Hygienic practices were also significantly related with the education level (χ² = 27.76, p <.001) and duration of vending (χ² = 41.44, p <.001). Better hygienic practices were observed in vendors who completed secondary school and those with long experience in the business. No meaningful relationship existed between prior food safety training and subsequent hygiene practice (χ²= 2.00, p =.368). Therefore, food safety improvement depends on ongoing practical training in food safety and reliable access to necessary infrastructure for maintaining hygiene including suitable food storage and preparation.

Abstract Nitrites are one of the multifunctional additives used in meat products. Given that they have high performance such as color creation, flavor improvement, and antioxidant and antimicrobial properties, they have caused consumer concern due to the formation of N-nitroso compounds and related health risks. Consumers demand natural or low-nitrite meat products. Synthetic nitrites are widely used because of their cheapness and easy access. However, due to the formation of nitrosamines, researchers are looking for a safer alternative to this additive. Plants, due to their nitrate content, can be used as natural nitrite sources by nitrate-reducing bacteria. Also, emerging non-thermal technologies such as atmospheric non-thermal plasma and pulsed electric fields can have the potential to be considered as new approaches for nitrite reduction. However, challenges regarding the stability of their sensory properties and antimicrobial efficacy remain. This review systematically evaluates the multifunctional role of nitrites, their health-related implications and potential substitution strategies, and addresses safe, effective, and acceptable solutions for producing high-quality meat and processed products with natural ingredients with a nitrite reduction approach.

Abstract Food additives, such as sweeteners and food colorants, play an important role in the food supply. But despite their widespread use in food, there are always concerns about their side effects on consumer health, and their excessive use can lead to adverse effects. This study aims to provide a brief overview of the beneficial and harmful effects of some sweeteners and food colorants on human health. For this purpose, studies conducted on these food additives from 2001 to 2025 were reviewed. Related articles were collected by using the keywords "food additives, sweeteners, colorants, and health effects." From scientific databases such as Pubmed, Science Direct, Elsevier, SID, etc. The results of studies have shown that artificial sweeteners and colorants, despite their widespread use in industry, can compromise food safety and lead to side effects such as gastrointestinal disorders, allergies, and even cancer in consumers. On the other hand, with the increase in consumer awareness, the demand for natural additives has increased. Therefore, dyes and artificial sweeteners should be limited, and their natural types should be used instead.

Abstract Postbiotics are metabolites derived from probiotics and other beneficial microorganisms that due to containing organic acids, bacteriocins, and bioactive peptides possess antimicrobial and antioxidant properties. In recent years, the use of postbiotics as a functional agent for food packaging systems has increased in popularity among researchers. However, although the postbiotics-packaging system has increased gradually, there are some obstacles that challenge it such as the safety and stability of novel beneficial microorganisms like mold as a next generation of postbiotics sources, parameters optimization of preparation and treatments of postbiotics, safety and performance of postbiotic carrier polymer and the sensorial effect of postbiotics on packaged foods. In addition, to ensure the consumption of packaged food with postbiotic-containing polymers, its safety needs to be thoroughly investigated, as research has shown that the postbiotic administration can have negative effects such as gastrointestinal complications, and allergies on the consumer body's health. Finally, for the entry of postbiotics as functional compounds into the food packaging market, the collaboration between academia and industry, clear labeling of packaged food and increasing consumer awareness about postbiotics are essential. Therefore, this study aims to briefly overview of the current status and future directions of postbiotics in food packaging.

Abstract Aflatoxin contamination poses a significant threat to agricultural systems and public health, particularly in regions such as Dodoma, where favorable climatic conditions promote the growth of aflatoxin-producing fungi. Various interventions were initiated to address the problem of aflatoxin contamination among maize farmers in Dodoma. This study aimed to assess maize farmers' knowledge, attitudes, and practices (KAP) following intervention programs designed to mitigate aflatoxin contamination in Chamwino district, Dodoma. A cross-sectional survey was conducted in five wards of Chamwino District: Chilonwa, Msanga, Chamwino, Majeleko, and Buigiri. A semi-structured questionnaire was administered to 300 maize farmers to assess their KAP related to aflatoxin contamination. Data were analyzed using SPSS version 26, with significance set at p ≤ 0.05. Among 300 farmers surveyed, 221 (73.7%) demonstrated awareness of aflatoxin-related interventions, whereas 79 (26.3%) did not exhibit such awareness. Of the 221 informed farmers, 123 (55.7%) demonstrated good knowledge, 51 (23.1%) exhibited medium knowledge, and 47 (21.3%) displayed poor knowledge regarding aflatoxin contamination and its mitigation. Of the participants, 186 (84.2%) exhibited positive attitudes, 34 (15.4%) displayed medium attitudes, and only 1 (0.5%) demonstrated poor attitudes. Of the participants, 157 (71.0%) exhibited good practices, while 64 (29.0%) were categorized as having medium-level practices. No farmers demonstrated poor practices regarding aflatoxin contamination and mitigation. The interventions effectively enhanced awareness, knowledge, attitudes, and practices regarding aflatoxin mitigation among maize farmers in Chamwino District. Future interventions should strengthen collaboration between government agencies, Non-Government Organizations, farmer groups, and local communities to sustain and expand these gains.

Abstract In Tanzania, tomatoes (Solanum lycopersicum) are widely cultivated and consumed, yet substantial postharvest losses occur due to their high perishability. Drying is a promising method to extend shelf life and add value to tomato products. This study evaluated the physicochemical, nutritional, microbiological, and sensory qualities of tomato soup powders produced using solar tunnel and electric dryers, stored in paper and plastic packaging at room temperature for five months. Two soup formulations were prepared using blends of dried tomato, garlic, onion, ginger, turmeric, cinnamon, monosodium glutamate, and cornstarch, differing in ingredient ratios. The pH, titratable acidity, total soluble solids, lycopene, β-carotene, vitamin C, and yeast and mold counts were measured to assess the effects of drying techniques, packaging materials, and storage duration. Results showed that drying method and packaging significantly influenced nutrient retention and microbial stability (p < 0.05). Soups dried with an electric dryer and packed in polyethylene retained higher nutrients, with lycopene decreasing from 17.35 to 13.12 mg/100 g, β-carotene from 5.20 to 3.85 mg/100 g, and vitamin C from 42.30 to 28.10 mg/100 g over five months. Microbial counts in polyethylene-packed samples remained below 10³ CFU/g after four months but exceeded limits in paper-packed samples. Sensory evaluation indicated both products were acceptable, with a slight preference for electric-dried soups. Overall, combining electric drying with polyethylene packaging effectively preserved the nutritional quality, microbial safety, and sensory acceptability of tomato soup powders for up to four months, offering a practical approach to reducing tomato postharvest losses.

Abstract Despite the relatively short history of the discovery of carbon dots (C-dots) and the development of their applications, methods for producing nanodots based on green chemistry have consistently attracted the attention of scientists. In this study, green C-dots were synthesized from onion juice using a simple hydrothermal method (200°C, 4 hours), and their optical properties, particle size distribution, and morphology were subsequently evaluated. The antibacterial activity of C-dots was assessed against pathogenic bacteria Escherichia coli (Gram-negative) and Listeria monocytogenes (Gram-positive), with minimum inhibitory concentrations of 8 mg mL-1 and 4 mg mL-1, respectively. Furthermore, the synthesized C-dots were incorporated into nanocellulose using an ex-situ method to produce modified bacterial nanocellulose (BNC) films with both antimicrobial and ultraviolet (UV) protective properties. The carbon dot-embedded nanocellulose demonstrated enhanced UV-blocking capabilities and greater inhibitory activity against Gram-positive bacteria compared to Gram-negative bacteria, highlighting its potential as a promising nano-biomaterial for food packaging applications.

Abstract Sesame (Sesamum indicum L.) is one of the most important oilseed crops cultivated worldwide. Sesame cake, as a by-product of sesame oil extraction contains approximately 50% protein. So, it has high potential for use as a protein source for a wide range of applications in the food industry. The sesame protein isolate (SPI), as a promising plant-based protein, can be used as a value-added hydrocolloid. Introducing the capabilities of the SPI can help expand its application in the food sector. This paper investigates those functional characteristics of the SPI that are important for use in food. The extraction, purification, and chemistry of the SPI are investigated. The solubility, water/oil binding capacity, rheological properties, and the ability to interact with polysaccharides are the most important characteristics of the SPI related to food application that are discussed in this paper. Moreover, all the potential applications of the SPI in the food industry are reviewed. This valuable plant protein has been used as an emulsifying/foaming agent, gelling hydrocolloid, film-forming/edible coating material, and also as a wall material for encapsulation purposes. The benefits and shortcomings of the SPI for all applications are discussed. The fields of use of the SPI in the food industry are much wider. This review paper is expected to open a new horizon in the use of this plant protein in the food industry by introducing the importance of SPI as a value-added by-product hydrocolloid.

Abstract Animal-derived proteins, mainly coming from meat, fish, and poultry, are vital for ensuring nutrition security, especially in many developing countries. However, poor handling practices throughout the supply chain pose serious risks of foodborne illnesses. This review explores the safety challenges related to the handling and processing of these items in developing nations. It highlights key shortcomings in pre-harvest management, such as weak veterinary oversight and limited disease control at the primary production stage. It also points out processing and distribution as potential contamination points, often worsened by poor temperature control, inconsistent application of Hazard Analysis Critical Control Point (HACCP) systems, and infrastructural issues. Globally, contaminated food causes about 10% of yearly illnesses, leading to roughly 420000 deaths. Therefore, strengthening quality and safety management systems at all stages—from production and processing to distribution and consumer handling—is crucial so stakeholders along the supply chain can effectively reduce these risks and protect public health. This review stresses the need for better veterinary services, improved disease management protocols, and strict safety practices to tackle these ongoing issues. Governments, companies, and NGOs should work on upgrading infrastructure, hygiene standards, and training, along with implementing regulations, to improve food safety management from farm to fork.