Food Safety and Packaging
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Changes in antibiotic susceptibility of Salmonella Typhimurium and Listeria monocytogenes under conditions of biofilm formation and freezing stress in meat juice model

Document Type : Original Article

Author

Ata Kaboudari

Department of Microbiology and Food Safety, Faculty of Veterinary Medicine, Lorestan University, Korramabad, Iran

https://doi.org/10.30466/fsp.2025.56711.1021
Abstract
The aim of this study was to investigate the changes in antibiotic susceptibility /resistance of bacteria isolated from biofilms in the presence of freezing stress in different meat juices (beef, mutton and goat). Gram-positive and Gram-negative bacteria of food origin (Listeria monocytogenes and Salmonella enterica ser. Typhimurium) were selected. Meat juice model was selected as a nutritious and complex environment containing a wide range of different compounds. Biofilms were studied in 96-well microplates and disk diffusion method was used to measure the antibiotic susceptibility. The results showed that the bacteria isolated from biofilms in meat juice models and freezing conditions exhibited significant changes in their antibiotic susceptibility. Long-term freezing (3 weeks and 1 month) significantly increased the antibiotic resistance. Both bacteria became resistant to chloramphenicol. The results indicated that foods should not be subjected to long-term freezing conditions so that existing pathogens do not acquire the ability to resist antibiotics.

Keywords

Antibiotic Resistance
Food Processing
Meat
Zoonoses
Subjects
Food Safety and Packaging
Volume 1, Issue 3
Autumn 2025
Pages 181-186

  • Receive Date 31 October 2025
  • Revise Date 12 November 2025
  • Accept Date 14 November 2025

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